发明公开
- 专利标题: ANIMAL CELL CULTURING METHOD
- 专利标题(中): TIERZELLEN-KULTIVIERUNGSVERFAHREN
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申请号: EP11854471.7申请日: 2011-12-28
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公开(公告)号: EP2660328A1公开(公告)日: 2013-11-06
- 发明人: KISHISHITA, Shohei , OKUI, Tomoko , SHINODA, Yasuharu , TAKUMA, Shinya
- 申请人: Chugai Seiyaku Kabushiki Kaisha
- 申请人地址: 5-1, Ukima 5-chome Kita-ku Tokyo 115-8543 JP
- 专利权人: Chugai Seiyaku Kabushiki Kaisha
- 当前专利权人: Chugai Seiyaku Kabushiki Kaisha
- 当前专利权人地址: 5-1, Ukima 5-chome Kita-ku Tokyo 115-8543 JP
- 代理机构: Vossius & Partner
- 优先权: JP2010291636 20101228
- 国际公布: WO2012091124 20120705
- 主分类号: C12P21/02
- IPC分类号: C12P21/02 ; C12P21/08 ; C12R1/91
摘要:
While a desired protein is prepared by culturing an animal cell that produces the protein to cause the protein to be produced, level of heterogeneity components of the protein is modulated by performing the culture at a normal culture temperature for a certain period and then continuing the culture at a culture temperature lowered to 25-35°C.
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