公开(公告)号：EP2660328A1

公开(公告)日：2013-11-06

申请号：EP11854471.7

申请日：2011-12-28

申请人： Chugai Seiyaku Kabushiki Kaisha

发明人： KISHISHITA, Shohei ,  OKUI, Tomoko ,  SHINODA, Yasuharu ,  TAKUMA, Shinya

IPC分类号： C12P21/02 ,  C12P21/08 ,  C12R1/91

CPC分类号： C07K16/28 ,  C07K16/244 ,  C07K16/2866 ,  C07K16/303 ,  C07K2317/14 ,  C12N5/0602 ,  C12N2511/00 ,  C12N2523/00 ,  C12P21/02

摘要： While a desired protein is prepared by culturing an animal cell that produces the protein to cause the protein to be produced, level of heterogeneity components of the protein is modulated by performing the culture at a normal culture temperature for a certain period and then continuing the culture at a culture temperature lowered to 25-35°C.

摘要翻译： 虽然通过培养产生蛋白质以产生蛋白质的动物细胞制备所需的蛋白质，但是通过在正常培养温度下进行一定时间的培养，然后继续培养来调节蛋白质的异质性成分的水平 在培养温度降至25-35℃。