发明公开
EP2751268A1 METHOD AND KIT FOR THE ISOLATION OF GENOMIC DNA, RNA, PROTEINS AND METABOLITES FROM A SINGLE BIOLOGICAL SAMPLE
有权
式分离方法基因组DNA，RNA，蛋白质和代谢物从生物样品中
- 专利标题： METHOD AND KIT FOR THE ISOLATION OF GENOMIC DNA, RNA, PROTEINS AND METABOLITES FROM A SINGLE BIOLOGICAL SAMPLE
- 专利标题（中）： 式分离方法基因组DNA，RNA，蛋白质和代谢物从生物样品中
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申请号： EP12745471.8申请日： 2012-08-02
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公开(公告)号： EP2751268A1公开(公告)日： 2014-07-09
- 发明人: WILMES, Paul , ROUME, Hugo , HILLER, Karsten , CORDES, Thekla
- 申请人： Université du Luxembourg , Centre de Recherche Public - Gabriel Lippmann
- 申请人地址： 162 A, Avenue de la Faïencerie 1511 Luxembourg LU
- 专利权人： Université du Luxembourg,Centre de Recherche Public - Gabriel Lippmann
- 当前专利权人： Université du Luxembourg,Centre de Recherche Public - Gabriel Lippmann
- 当前专利权人地址： 162 A, Avenue de la Faïencerie 1511 Luxembourg LU
- 代理机构： Lecomte & Partners
- 优先权： LU91864 20110902
- 国际公布： WO2013029919 20130307
- 主分类号： C12N15/10
- IPC分类号： C12N15/10
摘要：
The invention provides method and kit for the separation and purification of cellular components including polar and non-polar metabolites, genomic DNA, RNA and proteins from a single biological sample where two steps of lysis of the cells are performed sequentially, before and after a metabolite isolation step. The first lysis step is mechanical and performed in order to be incomplete, whereas the second is chemical or both mechanical and chemical. A sequential isolation of genomic DNA, RNA and proteins is carried out after the second lysis step.
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