发明授权
EP2821413B1 Nucleotide-specific recognition sequences for designer tal effectors
有权
对于设计师TAL效应Nucleotidspezifische识别序列
- 专利标题： Nucleotide-specific recognition sequences for designer tal effectors
- 专利标题（中）： 对于设计师TAL效应Nucleotidspezifische识别序列
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申请号： EP14179189.7申请日： 2012-11-30
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公开(公告)号： EP2821413B1公开(公告)日： 2016-09-07
- 发明人: Zhang, Feng , Cong, Le
- 申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
- 申请人地址： 7 Cambridge Center Cambridge, MA 02142 US
- 专利权人： The Broad Institute, Inc.,Massachusetts Institute of Technology,President and Fellows of Harvard College
- 当前专利权人： The Broad Institute, Inc.,Massachusetts Institute of Technology,President and Fellows of Harvard College
- 当前专利权人地址： 7 Cambridge Center Cambridge, MA 02142 US
- 代理机构： Cabinet Plasseraud
- 优先权： US201161565171P 20111130; US201213554922 20120720; US201213604945 20120906
- 主分类号： C07K14/03
- IPC分类号： C07K14/03 ; C12Q1/68 ; C07K14/195 ; C07K14/47 ; C12N5/07 ; C12N5/071 ; C12N9/12 ; C12N9/22 ; C12N15/63
摘要：
The invention relates to methods of altering expression of a genomic locus of interest or specifically targeting a genomic locus of interest in an animal cell, which may involve contacting the genomic locus with a non-naturally occurring or engineered composition that includes a deoxyribonucleic acid (DNA) binding polypeptide having a N-terminal capping region, a DNA binding domain comprising at least five or more Transcription activator-like effector (TALE) monomers and at least one or more half-monomers specifically ordered to target the genomic locus of interest, and a C-terminal capping region, wherein the polypeptide includes at least one or more effector domains, and wherein the polypeptide is encoded by and translated from a codon optimized nucleic acid molecule so that the polypeptide preferentially binds to the DNA of the genomic locus.
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