发明公开
EP2836607A1 DETECTION OF IMMUNOGLOBULIN LIGHT CHAIN RESTRICATION BY RNA IN SITU HYBRIDIZATION
有权
RESTRICTIONS EASIER免疫球蛋白检测中的RNA原位杂交
- 专利标题： DETECTION OF IMMUNOGLOBULIN LIGHT CHAIN RESTRICATION BY RNA IN SITU HYBRIDIZATION
- 专利标题（中）： RESTRICTIONS EASIER免疫球蛋白检测中的RNA原位杂交
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申请号： EP13772909.1申请日： 2013-04-05
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公开(公告)号： EP2836607A1公开(公告)日： 2015-02-18
- 发明人: MA, Xiao-Jun , LUO, Yuling , TUBBS, Raymond
- 申请人： Advanced Cell Diagnostics, Inc. , THE CLEVELAND CLINIC FOUNDATION
- 申请人地址： 3960 Point Eden Way Hayward, CA 94545 US
- 专利权人： Advanced Cell Diagnostics, Inc.,THE CLEVELAND CLINIC FOUNDATION
- 当前专利权人： Advanced Cell Diagnostics, Inc.,THE CLEVELAND CLINIC FOUNDATION
- 当前专利权人地址： 3960 Point Eden Way Hayward, CA 94545 US
- 代理机构： Jones Day
- 优先权： US201261620634P 20120405; US201261717064P 20121022; US201313843408 20130315
- 国际公布： WO2013152295 20131010
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The invention provides a method for detecting immunoglobulin light chain restriction and clonality in B cells by obtaining a sample of B cells from a subject; conducting a duplex in situ hybridization assay on the sample using (i) at least one probe set which is designed to specifically hybridize to immunoglobulin kappa chain constant region (IGKCR) RNA; and (ii) at least one probe set which is designed to specifically hybridize to immunoglobulin lambda chain constant region (IGLCR) RNA; detecting signal associated with hybridized IGKCR probe and signal associated with hybridized IGLCR probe in a population of B cells in the sample; and determining a pattern of signal associated with hybridized IGKCR probe and hybridized IGLCR probe within individual cells in the B cell population, wherein the pattern of signal within individual cells indicates the presence or absence of light chain restriction and clonality of the B cells.
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