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    BIOMARKERS FOR DIFFERENTIATING MELANOMA FROM BENIGN NEVUS IN THE SKIN
    3.
    发明授权
    BIOMARKERS FOR DIFFERENTIATING MELANOMA FROM BENIGN NEVUS IN THE SKIN 有权

    公开(公告)号：EP2619321B1

    公开(公告)日：2018-08-01

    申请号：EP11764045.8

    申请日：2011-09-20

    申请人： Advanced Cell Diagnostics, Inc.

    发明人： MA, Xiao-Jun ,  WU, Xingyong ,  LUO, Yuling

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16

    摘要： Disclosed is a method for diagnosing melanoma in a human subject, as well as a method for providing a prognosis to a human subject who is at risk of developing melanoma recurrence, and a method for determining the stage of melanoma in a human subject, comprising the step of determining the level of expression of phosphatase and actin regulator 1 (PHACTR1) gene, or fragments thereof, either alone or in combination with the level of expression of secreted integrin-binding phosphoprotein (SPP1), preferentially expressed antigen in melanoma (PRAME), growth differentiation factor 15 (GDF 15), and chemokine C-X-C motif ligand 10 (CXCL10) genes. Further, the invention relates to a diagnostic kit, comprising at least one substance for detection of the expression of PHACTR1, or fragments thereof, either alone or in combination with the detection of SPP1, PRAME, GDF15, and CXCL10, for the diagnosis or prognosis of melanoma.

    METHODS OF DETECTING NUCLEIC ACIDS IN INDIVIDUAL CELLS AND OF IDENTIFYING RARE CELLS FROM LARGE HETEROGENEOUS CELL POPULATIONS
    6.
    发明公开
    METHODS OF DETECTING NUCLEIC ACIDS IN INDIVIDUAL CELLS AND OF IDENTIFYING RARE CELLS FROM LARGE HETEROGENEOUS CELL POPULATIONS 审中-公开
    方法对于核酸的单个细胞检测和确定稀有细胞从大型异质细胞群

    公开(公告)号：EP3042963A1

    公开(公告)日：2016-07-13

    申请号：EP15194063.2

    申请日：2006-06-19

    申请人： Advanced Cell Diagnostics, Inc.

    发明人： Luo, Yuling ,  Chen, Shiping

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  B01L3/00

    CPC分类号： C12Q1/6837 ,  C12Q1/682 ,  C12Q1/6841 ,  C12Q2537/143 ,  C12Q2537/125 ,  C12Q2565/543 ,  C12Q2525/313

    摘要： The invention provides a method of detecting a nucleic acid target within a cell in a sample, the method comprising labeling the nucleic acid using at least two capture probes that capture a label probe to the nucleic acid target. The signal may be amplified with a help of one or more amplifiers and preamplifiers. The method is useful inter alia for identifying a cell of a certain type in a mixture of cells based. The invention further provides a kit for carrying out the method of the invention as well as a composition and a tissue section comprising a fixed and permeabilized cell with the labeling system as identified for the method of the invention.

    摘要翻译： 本发明提供了样品中的细胞内检测的核酸靶的方法，其包括标记使用至少两个捕获探针的核酸的方法所做捕获标记探针与核酸靶标。 该信号可以与一个或多个放大器和前置放大器的帮助下被放大。 该方法是用于基于细胞的混合物识别特定类型的细胞中有用尤其。 本发明还提供用于实施本发明的方法以及组合物和组织切片包括与标记系统的固定和透化细胞作为确定了本发明的方法的试剂盒。

    DETECTION OF IMMUNOGLOBULIN LIGHT CHAIN RESTRICATION BY RNA IN SITU HYBRIDIZATION
    9.
    发明公开
    DETECTION OF IMMUNOGLOBULIN LIGHT CHAIN RESTRICATION BY RNA IN SITU HYBRIDIZATION 有权
    原位杂交技术鉴定轻链免疫球蛋白链

    公开(公告)号：EP2836607A4

    公开(公告)日：2015-12-02

    申请号：EP13772909

    申请日：2013-04-05

    申请人： ADVANCED CELL DIAGNOSTICS INC ,  CLEVELAND CLINIC FOUNDATION

    发明人： MA XIAO-JUN ,  LUO YULING ,  TUBBS RAYMOND

    IPC分类号： C12Q1/68 ,  C07K16/00

    CPC分类号： C12Q1/6881 ,  C07K16/00 ,  C07K2317/515 ,  C12Q1/6886 ,  C12Q2600/158

    摘要： The invention provides a method for detecting immunoglobulin light chain restriction and clonality in B cells by RNA in situ hybridization assays on a sample of B cells from a subject. The detection is based on the pattern of signal associated with hybridized immunoglobulin kappa chain constant region (IGKCR) probe, immunoglobulin lambda chain constant region (IGLCR) probe, and immunoglobulin lambda-like polypeptide 5 (IGLL5) probe. The invention also provides a kit for conducting the RNA in situ hybridization assays.

    摘要翻译： 本发明提供一种通过从受试者获得B细胞样品来检测B细胞中免疫球蛋白轻链限制和克隆性的方法; 使用（i）至少一个被设计为与免疫球蛋白κ恒定区（IGKCR）RNA特异性杂交的探针组，对样品进行双相原位杂交测定; 和（ii）设计为与免疫球蛋白λ链恒定区（IGLCR）RNA特异性杂交的至少一个探针组; 检测与杂交的IGKCR探针相关的信号和与样品中B细胞群中的杂交IGLCR探针相关的信号; 以及确定与B细胞群体中各个细胞内的杂交IGKCR探针和杂交IGLCR探针相关联的信号模式，其中各个细胞内的信号模式指示存在或不存在B细胞的轻链限制和克隆。