发明公开
EP2844392A1 MODULES AND CALIBRATION METHOD FOR A ROBOTIC POLYNUCLEOTIDE SAMPLE PREPARATION SYSTEM
审中-公开
模块和一个机器人POLYNUKLEOTIDPROBENHERSTELLUNGSSYSTEM方法
- 专利标题: MODULES AND CALIBRATION METHOD FOR A ROBOTIC POLYNUCLEOTIDE SAMPLE PREPARATION SYSTEM
- 专利标题(中): 模块和一个机器人POLYNUKLEOTIDPROBENHERSTELLUNGSSYSTEM方法
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申请号: EP13713643.8申请日: 2013-03-14
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公开(公告)号: EP2844392A1公开(公告)日: 2015-03-11
- 发明人: BARBEE, Kristopher , JONES, Ryan , MCCUSKER, Sean , CARPINO, Maximilian , LEAMON, John , SCHULTZ, Jonathan , HOSHIZAKI, Jon
- 申请人: Life Technologies Corporation
- 申请人地址: 5791 Van Allen Way Carlsbad, CA 92008 US
- 专利权人: Life Technologies Corporation
- 当前专利权人: Life Technologies Corporation
- 当前专利权人地址: 5791 Van Allen Way Carlsbad, CA 92008 US
- 代理机构: Wöhler, Christian
- 优先权: US201261640243P 20120430; US201261668938P 20120706; US201261700003P 20120912
- 国际公布: WO2013165594 20131107
- 主分类号: B01L7/00
- IPC分类号: B01L7/00 ; C12Q1/68 ; G01N35/10 ; G01N35/00
摘要:
An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a centrifuge for breaking the emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. A method for calibrating the z-position of a translation device moving a pipette Hp is included. An imaging system is used for assessing attachement of pipette tip.
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