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公开(公告)号：EP3347129A1
公开(公告)日：2018-07-18
申请号：EP16770826.2
申请日：2016-09-08
发明人： ROSWECH, Todd , SCHULTZ, Jonathan , HO, Chun
CPC分类号： B67D7/0266 , B01L3/502 , B01L3/523 , B01L3/563 , B01L2200/026 , B01L2200/0684 , B01L2200/0689 , B01L2300/043 , B01L2400/0487 , B67D7/0288 , B67D7/76 , B67D2210/0001 , G01N35/1002
摘要： A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.
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公开(公告)号：EP2952590B1
公开(公告)日：2017-07-26
申请号：EP15178097.0
申请日：2011-06-10
CPC分类号： C12Q1/6869 , B01L3/5027 , C12Q1/6874 , C12Q2537/155 , C12Q2563/149 , C12Q2565/301 , C12Q2565/629
摘要： A method of sequencing a polynucleotide strand may include following operations: (i) providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand, and (ii) successively exposing the polynucleotide strand to the flow of four different dNTPs according to a predetermined ordering. The predetermined ordering may include an alternate ordering which is not a continuous repeat of an ordering of the four different dNTPs.
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公开(公告)号：EP3582224A1
公开(公告)日：2019-12-18
申请号：EP19162210.9
申请日：2011-12-29
发明人： REARICK, Todd , SCHULTZ, Jonathan
IPC分类号： G16B40/10 , G16B30/20 , C12Q1/6869
摘要： Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled.
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4.发明公开MODULES AND CALIBRATION METHOD FOR A ROBOTIC POLYNUCLEOTIDE SAMPLE PREPARATION SYSTEM 审中-公开模块和一个机器人POLYNUKLEOTIDPROBENHERSTELLUNGSSYSTEM方法
公开(公告)号：EP2844392A1
公开(公告)日：2015-03-11
申请号：EP13713643.8
申请日：2013-03-14
发明人： BARBEE, Kristopher , JONES, Ryan , MCCUSKER, Sean , CARPINO, Maximilian , LEAMON, John , SCHULTZ, Jonathan , HOSHIZAKI, Jon
CPC分类号： C12Q1/6806 , B04B5/0421 , B04B13/00 , C12Q1/6869 , G01N35/00693 , G01N35/1011 , G01N2035/00495 , G01N2035/1013 , G01N2035/103
摘要： An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a centrifuge for breaking the emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. A method for calibrating the z-position of a translation device moving a pipette Hp is included. An imaging system is used for assessing attachement of pipette tip.
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公开(公告)号：EP2658999A2
公开(公告)日：2013-11-06
申请号：EP11811287.9
申请日：2011-12-29
发明人： REARICK, Todd , SCHULTZ, Jonathan
CPC分类号： G06F19/24 , C12Q1/6869 , C12Q2527/119 , C12Q2563/159 , C12Q2565/607
摘要： Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled.
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公开(公告)号：EP2321434A2
公开(公告)日：2011-05-18
申请号：EP09805290.5
申请日：2009-08-07
发明人： ROTHBERG, Jonathan, M. , LEAMON, John, H. , DAVIDSON, John, F. , VAN OIJEN, Antoine, M. , HINZ, Wolfgang , DAVEY, Melville , HANN, Bradley , SCHULTZ, Jonathan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2523/303 , C12Q2565/131 , C12Q2525/204 , C12Q2521/101 , C12Q2535/122
摘要： The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.
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公开(公告)号：EP2658999B1
公开(公告)日：2019-03-13
申请号：EP11811287.9
申请日：2011-12-29
发明人： REARICK, Todd , SCHULTZ, Jonathan
IPC分类号： G06F19/22 , G06F19/24 , C12Q1/6869
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8.发明授权
公开(公告)号：EP2694675B1
公开(公告)日：2018-01-24
申请号：EP12713843.6
申请日：2012-04-05
发明人： HUBBELL, Earl , SCHULTZ, Jonathan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2533/101 , C12Q2565/301 , C12Q2565/607
摘要： A system and machine readable medium for nucleic acid sequencing includes disposing template polynucleotide strands in defined spaces disposed on a sensor array, at least some of the template polynucleotide strands having a sequencing primer and a polymerase operably bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed according to a predetermined ordering; and determining, for each of the series of flows of nucleotide species, how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands, wherein the predetermined ordering (a) is not a series of consecutive repetitions of a 4-flow permutation of four different nucleotide species, (b) is not specifically tailored to a particular combination of a particular template polynucleotide strand to be sequenced and a particular sequencing primer to be used, and (c) comprises a phase-protecting flow ordering.
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公开(公告)号：EP3154695A1
公开(公告)日：2017-04-19
申请号：EP15744390.4
申请日：2015-06-15
发明人： SCHULTZ, Jonathan , ROSWECH, Todd
CPC分类号： B01L3/502 , B01F11/0008 , B01F11/0065 , B01F15/0238 , B01F15/028 , B01L3/505 , B01L3/527 , B01L2200/04 , B01L2200/16 , B01L2300/022 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B65D81/3272 , G01N35/1002 , Y10T436/2575
摘要： An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
摘要翻译： 用于制备试剂溶液的装置包括外壳和设置在外壳内的容器。 容器限定具有可压缩体积的内腔，并且限定了在内腔和容器的外部之间提供流体连通的通道。 可选地，可压缩构件设置在内部空腔内。 试剂设置在内腔内。
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10.发明公开
公开(公告)号：EP2694675A1
公开(公告)日：2014-02-12
申请号：EP12713843.6
申请日：2012-04-05
发明人： HUBBELL, Earl , SCHULTZ, Jonathan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2533/101 , C12Q2565/301 , C12Q2565/607
摘要： A system and machine readable medium for nucleic acid sequencing includes disposing template polynucleotide strands in defined spaces disposed on a sensor array, at least some of the template polynucleotide strands having a sequencing primer and a polymerase operably bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed according to a predetermined ordering; and determining, for each of the series of flows of nucleotide species, how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands, wherein the predetermined ordering (a) is not a series of consecutive repetitions of a 4-flow permutation of four different nucleotide species, (b) is not specifically tailored to a particular combination of a particular template polynucleotide strand to be sequenced and a particular sequencing primer to be used, and (c) comprises a phase-protecting flow ordering.
摘要翻译： 用于核酸测序的方法包括将多个模板多核苷酸链放置在设置在传感器阵列上的多个限定空间中，至少一些模板多核苷酸链具有测序引物和与其可操作地结合的聚合酶; 用测序引物和与之可操作地结合的聚合酶将模板多核苷酸链暴露于按照预定顺序流动的一系列核苷酸物质流; 并且针对核苷酸物种的一系列流的每一个确定针对该特定流发生了多少核苷酸掺入以确定对应于模板多核苷酸链的预测核苷酸序列，其中预定排序（a）不是一系列连续的 （b）不是专门针对待测序的特定模板多核苷酸链和待使用的特定测序引物的特定组合，并且（c）包含相变材料， 保护流量排序。
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