- 专利标题: METHODS FOR NUCLEIC ACID ASSEMBLY AND HIGH THROUGHPUT SEQUENCING
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申请号: EP13809969.2申请日: 2013-06-24
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公开(公告)号: EP2864531B1公开(公告)日: 2018-10-24
- 发明人: HUDSON, Michael, E. , KUNG, Li-yun A. , SCHINDLER, Daniel , ARCHER, Stephen , SAAEM, Ishtiaq
- 申请人: Gen9, Inc.
- 申请人地址: 27 Drydock Avenue, 8th Floor Boston MA 02210 US
- 专利权人: Gen9, Inc.
- 当前专利权人: Gen9, Inc.
- 当前专利权人地址: 27 Drydock Avenue, 8th Floor Boston MA 02210 US
- 代理机构: Sutcliffe, Nicholas Robert
- 优先权: US201261664118P 20120625; US201261731627P 20121130
- 国际公布: WO2014004393 20140103
- 主分类号: C40B50/06
- IPC分类号: C40B50/06 ; C12N15/10 ; C12N15/66
摘要:
Methods and apparatus of some aspects of the invention relate to the synthesis of high fidelity polynucleotides. In particular, aspects of the invention relate to concurrent enzymatic removal of amplification sequences and ligation of processed oligonucleotides into nucleic acid assemblies. According to some embodiments, the invention provides a method for producing a target nucleic acid having a predefined sequence. In some embodiments, the method comprises the step of providing a plurality of oligonucleotides, wherein each oligonucleotides comprises (i) an internal sequence identical to a different portion of a sequence of a target nucleic acid, (ii) a 5′ sequence flanking the 5′ end of the internal sequence and a 3′ flanking sequence flanking the 3′ end of the internal sequence, each of the flanking sequence comprising a primer recognition site for a primer pair and a restriction enzyme recognition site.
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