发明公开
EP2883051A1 IMMUNOASSAY-BASED DETERMINATION OF IN-SOLUTION BINDING KINETICS
有权
LÖSUNGEN的免费BASTIMMUNG DER BINDUNGSKINETIK
- 专利标题： IMMUNOASSAY-BASED DETERMINATION OF IN-SOLUTION BINDING KINETICS
- 专利标题（中）： LÖSUNGEN的免费BASTIMMUNG DER BINDUNGSKINETIK
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申请号： EP13745066.4申请日： 2013-08-02
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公开(公告)号： EP2883051A1公开(公告)日： 2015-06-17
- 发明人: DAHL, Uwe , JORDAN, Gregor , STAACK, Roland
- 申请人： F.Hoffmann-La Roche AG
- 申请人地址： Grenzacherstrasse 124 4070 Basel CH
- 专利权人： F.Hoffmann-La Roche AG
- 当前专利权人： F.Hoffmann-La Roche AG
- 当前专利权人地址： Grenzacherstrasse 124 4070 Basel CH
- 代理机构： Burger, Alexander
- 优先权： EP12179742 20120808
- 国际公布： WO2014023655 20140213
- 主分类号： G01N33/557
- IPC分类号： G01N33/557
摘要：
Herein is reported a method for the determination of the binding affinity of a binder and its ligand comprising the step of determining based on the result of an immunoassay the fraction of free binder in a sample comprising binder, ligand and binder-ligand -complexes for at least two different binder:ligand ratios in the sample, and if the determined fraction of free binder is not comparable for all used binder:ligand ratios then the binder:ligand ratio in the sample is lowered and the sample is re-analyzed by the same immunoassay, and calculating based on the fraction of free binder in the previous step the binding affinity for the binder to its ligand.
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