发明公开
- 专利标题: METHOD FOR USING HEAT-RESISTANT MISMATCH ENDONUCLEASE
- 专利标题(中): 使用耐热性不匹配核酸内切酶的方法
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申请号: EP14762697.2申请日: 2014-03-13
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公开(公告)号: EP2975124A1公开(公告)日: 2016-01-20
- 发明人: MATSUMURA, Kiyoyuki , TAKATSU, Nariaki , UEMORI, Takashi , MUKAI, Hiroyuki
- 申请人: TAKARA BIO INC.
- 申请人地址: 4-1, Seta 3-chome Otsu-shi, Shiga 520-2193 JP
- 专利权人: TAKARA BIO INC.
- 当前专利权人: TAKARA BIO INC.
- 当前专利权人地址: 4-1, Seta 3-chome Otsu-shi, Shiga 520-2193 JP
- 代理机构: Schnappauf, Georg
- 优先权: JP2013052265 20130314
- 国际公布: WO2014142261 20140918
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C12N9/22 ; C12Q1/68
摘要:
Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
公开/授权文献
- EP2975124B1 METHOD FOR USING HEAT-RESISTANT MISMATCH ENDONUCLEASE 公开/授权日:2019-03-06
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