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公开(公告)号:EP2975124A1
公开(公告)日:2016-01-20
申请号:EP14762697.2
申请日:2014-03-13
申请人: TAKARA BIO INC.
CPC分类号: C12N9/22 , C12P19/34 , C12Q1/683 , C12Y301/21 , C12Q2521/301 , C12Q2521/514
摘要: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
摘要翻译: 提供了使用新型耐热错配核酸酶的错配特异性切割反应,使用错配核酸酶消除核酸扩增反应中错误的方法,抑制具有特定碱基序列的核酸在 核酸扩增反应,以及使用该抑制方法检测具有单碱基多态性突变的核酸的方法。
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公开(公告)号:EP2811020B1
公开(公告)日:2018-06-27
申请号:EP13743868.5
申请日:2013-01-24
申请人: Takara Bio Inc.
CPC分类号: C12Q1/6848 , C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Y207/07007 , C12Q2527/125
摘要: Provided are the following: a method, for improving reactivity of an acid synthesis reaction, comprising a step for adding an É-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an É-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an É-amino acid.
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公开(公告)号:EP2811020A1
公开(公告)日:2014-12-10
申请号:EP13743868.5
申请日:2013-01-24
申请人: Takara Bio, Inc.
CPC分类号: C12Q1/6848 , C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/6844 , C12Y207/07007 , C12Q2527/125
摘要: Provided are the following: a method, for improving reactivity of an acid synthesis reaction, comprising a step for adding an ω-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleotide triphosphate, and an ω-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ω-amino acid.
摘要翻译: 本发明提供一种提高酸合成反应的反应性的方法,其包括向反应液中添加ω-氨基酸的工序, 用于核酸合成反应的组合物,其包含DNA聚合酶,反应缓冲液,至少一种引物,至少一种脱氧核糖核苷酸三磷酸和ω-氨基酸; 和用于核酸合成反应的包含ω-氨基酸的反应缓冲液。
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