发明公开
- 专利标题： Improved nanopore plasmonic analyser
- 专利标题（中）： 改进的纳米孔电浆分析仪
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申请号： EP14184817.6申请日： 2014-09-15
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公开(公告)号： EP2995933A1公开(公告)日： 2016-03-16
- 发明人: The designation of the inventor has not yet been filed
- 申请人： Base4 Innovation Ltd
- 申请人地址： Broers Building J J Thomson Avenue Cambridge, Cambridgeshire CB3 0FA GB
- 专利权人： Base4 Innovation Ltd
- 当前专利权人： Base4 Innovation Ltd
- 当前专利权人地址： Broers Building J J Thomson Avenue Cambridge, Cambridgeshire CB3 0FA GB
- 代理机构： Lau, Sarah Jane
- 主分类号： G01N21/65
- IPC分类号： G01N21/65 ; B82Y5/00 ; B82Y15/00 ; B82Y35/00 ; C12Q1/68 ; G01J3/44 ; G01N33/487 ; G02B6/122
摘要：
An apparatus for investigating a molecule comprises:
• a substrate;
• first and second chambers arranged either side of the substrate;
• at least one nanopore in said substrate linking the first and second chambers;
• at least one pair of nanostructures, located on the substrate, capable of generating an electromagnetic field when caused to undergo localised surface plasmon resonance;
• a source of incident electromagnetic radiation for inducing localised surface plasmon resonance in the nanostructures:
• a controller for causing the molecule to pass from the first to the second chambers via the inlet(s) and outlet(s) and through the electromagnetic field and
• a detector for detecting Raman-scattered radiation produced by the molecules as they pass through the electromagnetic field.
It is characterised in that the nanostructures are juxtaposed opposite each other around the nanopore and have morphology in a plane parallel to that of the substrate comprising sectors of a circle having a from 30 to 150 degree central angle. The apparatus is especially suitable for investigating biomolecules; in particular for sequencing the amino acids of a protein or the nucleotides of a nucleic acid such as naturally occurring or synthetic RNA or DNA.
• a substrate;
• first and second chambers arranged either side of the substrate;
• at least one nanopore in said substrate linking the first and second chambers;
• at least one pair of nanostructures, located on the substrate, capable of generating an electromagnetic field when caused to undergo localised surface plasmon resonance;
• a source of incident electromagnetic radiation for inducing localised surface plasmon resonance in the nanostructures:
• a controller for causing the molecule to pass from the first to the second chambers via the inlet(s) and outlet(s) and through the electromagnetic field and
• a detector for detecting Raman-scattered radiation produced by the molecules as they pass through the electromagnetic field.
It is characterised in that the nanostructures are juxtaposed opposite each other around the nanopore and have morphology in a plane parallel to that of the substrate comprising sectors of a circle having a from 30 to 150 degree central angle. The apparatus is especially suitable for investigating biomolecules; in particular for sequencing the amino acids of a protein or the nucleotides of a nucleic acid such as naturally occurring or synthetic RNA or DNA.
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