发明公开
- 专利标题: PRODUCTION OF GLYCOPROTEINS HAVING INCREASED N-GLYCOSYLATION SITE OCCUPANCY
- 专利标题(中): 枸杞子
-
申请号: EP14738496.0申请日: 2014-07-10
-
公开(公告)号: EP3019621A1公开(公告)日: 2016-05-18
- 发明人: NATUNEN, Jari , LANDOWSKI, Christopher , SALOHEIMO, Markku , OSTERMEIER, Christian , SOMMER, Benjamin Patrick , WAHL, Ramon
- 申请人: Novartis AG , Glykos Finland Oy
- 申请人地址: Lichtstrasse 35 4056 Basel CH
- 专利权人: Novartis AG,Glykos Finland Oy
- 当前专利权人: Novartis AG,Glykos Finland Oy
- 当前专利权人地址: Lichtstrasse 35 4056 Basel CH
- 代理机构: Cabinet Plasseraud
- 优先权: EP13175997 20130710
- 国际公布: WO2015004239 20150115
- 主分类号: C12P21/00
- IPC分类号: C12P21/00 ; C12N9/10
摘要:
The present disclosure relates to compositions and methods useful for the production of heterologous proteins with increased N-glycosylation site occupancy in filamentous fungal cells, such as Trichoderma cells. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutation that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s), ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase, and iii. a polynucleotide encoding a heterologous glycoprotein, wherein said catalytic subunit of oligosaccharyl transferase is selected from Leishmania oligosaccharyl transferase catalytic subunits.
公开/授权文献
信息查询