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    PRODUCTION OF GLYCOPROTEINS HAVING INCREASED N-GLYCOSYLATION SITE OCCUPANCY
    1.
    发明公开
    PRODUCTION OF GLYCOPROTEINS HAVING INCREASED N-GLYCOSYLATION SITE OCCUPANCY 审中-公开
    枸杞子

    公开(公告)号：EP3019621A1

    公开(公告)日：2016-05-18

    申请号：EP14738496.0

    申请日：2014-07-10

    申请人： Novartis AG ,  Glykos Finland Oy

    发明人： NATUNEN, Jari ,  LANDOWSKI, Christopher ,  SALOHEIMO, Markku ,  OSTERMEIER, Christian ,  SOMMER, Benjamin Patrick ,  WAHL, Ramon

    IPC分类号： C12P21/00 ,  C12N9/10

    CPC分类号： C12P21/005 ,  C07K16/00 ,  C12N9/1051 ,  C12N9/1081 ,  C12N15/80 ,  C12Y204/01

    摘要： The present disclosure relates to compositions and methods useful for the production of heterologous proteins with increased N-glycosylation site occupancy in filamentous fungal cells, such as Trichoderma cells. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutation that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s), ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase, and iii. a polynucleotide encoding a heterologous glycoprotein, wherein said catalytic subunit of oligosaccharyl transferase is selected from Leishmania oligosaccharyl transferase catalytic subunits.

    摘要翻译： 本公开内容涉及用于生产丝状真菌细胞如木霉属细胞中具有增加的N-糖基化位点占据的异源蛋白质的组合物和方法。 更具体地，本发明提供丝状真菌细胞，其包含i。 一种或多种与不具有所述突变的亲本丝状真菌细胞相比减少或消除一种或多种内源蛋白酶活性的突变，ii。 编码寡糖基转移酶的异源催化亚基的多核苷酸，和iii。 编码异源糖蛋白的多核苷酸，其中所述寡糖基转移酶的催化亚单位选自利什曼原虫寡糖基转移酶催化亚基。

    PRODUCTION OF GLYCOPROTEINS WITH MAMMALIAN-LIKE N-GLYCANS IN FILAMENTOUS FUNGI
    10.
    发明公开
    PRODUCTION OF GLYCOPROTEINS WITH MAMMALIAN-LIKE N-GLYCANS IN FILAMENTOUS FUNGI 审中-公开
    哺乳动物丝状真菌中哺乳动物样N-糖苷生产糖蛋白

    公开(公告)号：EP3172333A1

    公开(公告)日：2017-05-31

    申请号：EP15738703.6

    申请日：2015-07-21

    申请人： Glykos Finland Oy

    发明人： NATUNEN, Jari ,  LEPPÄNEN, Anne ,  SALMINEN, Heidi ,  HILTUNEN, Jukka ,  KANERVA, Anne ,  HEISKANEN, Annamari ,  WESTERHOLM-PARVINEN, Ann ,  SCHMIDT, Georg ,  HUUSKONEN, Anne ,  MUSTALAHTI, Eero ,  LANDOWSKI, Christopher ,  SALOHEIMO, Markku ,  SAARINEN, Juhani ,  SOMMER, Benjamin Patrick ,  WAHL, Ramon ,  OSTERMEIER, Christian ,  HELK, Bernhard

    IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N9/24

    摘要： The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such as Trichoderma cells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii.a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing α1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.

    摘要翻译： 本公开涉及用于在丝状真菌细胞例如木霉属细胞中产生重组糖蛋白的组合物和方法，其中所述产生的全部中性N-聚糖的至少90％（mol％），优选至少95％ 重组糖蛋白是哺乳动物样N-聚糖。 更具体地说，本发明提供了一种丝状真菌细胞，其包含i。 与不具有所述突变的亲本丝状真菌细胞相比，减少或消除一种或多种内源性蛋白酶活性的一种或多种突变; ii。编码寡糖基转移酶的异源催化亚基的多核苷酸; III。 用于增加α1,2甘露糖苷酶活性的重组多核苷酸;和iv。 编码所述异源糖蛋白的重组多核苷酸。