发明公开
- 专利标题： MAMMALIAN EXPRESSION VECTOR
- 专利标题（中）： 加拿大梭鲈，EXPRESSIONSVEKTOR
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申请号： EP16168059.0申请日： 2008-12-18
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公开(公告)号： EP3075858A1公开(公告)日： 2016-10-05
- 发明人: KNOPF, Hans-Peter , WILMS, Burkhard
- 申请人： NOVARTIS AG
- 申请人地址： Lichtstrasse 35 4056 Basel CH
- 专利权人： NOVARTIS AG
- 当前专利权人： NOVARTIS AG
- 当前专利权人地址： Lichtstrasse 35 4056 Basel CH
- 代理机构： Roth, Carla
- 优先权： EP07150339 20071221
- 主分类号： C12N15/79
- IPC分类号： C12N15/79
摘要：
The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising
(a) at least one expression cassette (POI) for expressing a polypeptide of interest;
(b) an expression cassette (MSM) comprising a mammalian selectable marker gene;
(c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene;
wherein the expression cassette (POI) is flanked 5' by the expression cassette (MASM), the expression cassette (MSM) is located 3' from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5' to 3' orientation.
Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells.
(a) at least one expression cassette (POI) for expressing a polypeptide of interest;
(b) an expression cassette (MSM) comprising a mammalian selectable marker gene;
(c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene;
wherein the expression cassette (POI) is flanked 5' by the expression cassette (MASM), the expression cassette (MSM) is located 3' from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5' to 3' orientation.
Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells.
公开/授权文献
- EP3075858B1 MAMMALIAN EXPRESSION VECTOR 公开/授权日：2020-01-29
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