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    EXPRESSION VECTOR SYSTEM COMPRISING TWO SELECTION MARKERS
    5.
    发明公开
    EXPRESSION VECTOR SYSTEM COMPRISING TWO SELECTION MARKERS 有权
    表达载体系统中的两个选择标记

    公开(公告)号：EP2401377A1

    公开(公告)日：2012-01-04

    申请号：EP10711001.7

    申请日：2010-02-26

    申请人： Novartis AG

    发明人： JOSTOCK, Thomas ,  KNOPF, Hans-Peter

    IPC分类号： C12N15/79

    CPC分类号： C12N15/85 ,  C12N1/20 ,  C12N9/003 ,  C12N15/52 ,  C12N15/79 ,  C12N2800/40 ,  C12P21/02 ,  C12Y105/01003

    摘要： The invention pertains to an expression vector or a combination of at least two expression vectors comprising at least (a) a polynucleotide encoding a product of interest or an insertion site for incorporating a polynucleotide encoding a product of interest; (b) a polynucleotide encoding a first selectable marker (sm I); (c) a polynucleotide encoding a second selectable marker (sm II), which is different from the first selectable marker (sm I), wherein the activity of the selectable marker (sm I) or (sm II) is at least partially influenced by the activity of the other selectable marker and wherein the selectable markers (sm I) and (sm II) are involved in the folate metabolism. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.

    MAMMALIAN EXPRESSION VECTOR
    7.
    发明公开
    MAMMALIAN EXPRESSION VECTOR 审中-公开
    加拿大梭鲈，EXPRESSIONSVEKTOR

    公开(公告)号：EP3075858A1

    公开(公告)日：2016-10-05

    申请号：EP16168059.0

    申请日：2008-12-18

    申请人： NOVARTIS AG

    发明人： KNOPF, Hans-Peter ,  WILMS, Burkhard

    IPC分类号： C12N15/79

    摘要： The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising
    (a) at least one expression cassette (POI) for expressing a polypeptide of interest;
    (b) an expression cassette (MSM) comprising a mammalian selectable marker gene;
    (c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene;
    wherein the expression cassette (POI) is flanked 5' by the expression cassette (MASM), the expression cassette (MSM) is located 3' from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5' to 3' orientation.
    Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells.

    摘要翻译： 本发明提供用于在哺乳动物细胞中表达至少一种目的多肽的载体核酸，其包含（a）用于表达目的多肽的至少一种表达盒（POI）; （b）包含哺乳动物选择标记基因的表达盒（MSM）; （c）包含哺乳动物可扩增的可选择标记基因的表达盒（MASM）; 其中表达盒（POI）通过表达盒（MASM）在5'侧翼，表达盒（MSM）位于表达盒（POI）3'，其中表达盒（MASM），（POI）和（ MSM）以相同的5'至3'方向排列。 还提供了包含所述载体的宿主细胞和使用各自宿主细胞产生多肽的方法。

    CELL SURFACE DISPLAY OF POLYPEPTIDE ISOFORMS BY STOP CODON READTHROUGH
    8.
    发明公开
    CELL SURFACE DISPLAY OF POLYPEPTIDE ISOFORMS BY STOP CODON READTHROUGH 有权
    演示BY POLYPEPTIDISOFORMEN停止细胞表面密码子通读

    公开(公告)号：EP2329020A1

    公开(公告)日：2011-06-08

    申请号：EP09778177.7

    申请日：2009-08-28

    申请人： Novartis AG

    发明人： JOSTOCK, Thomas ,  KNOPF, Hans-Peter ,  WILMS, Burkhard ,  NOMMAY, Audrey Josiane

    IPC分类号： C12N15/10 ,  C12N15/62

    CPC分类号： C12N15/1037 ,  C07K2319/035

    摘要： The present invention inter alia pertains to a method for generating or selecting a eukaryotic host cell expressing a desired level of a polypeptide of interest, comprising: a) providing a plurality of eukaryotic host cells comprising a heterologous nucleic acid comprising at least one cassette (Cas-POI) comprising at least a first polynucleotide (Pn-POI) encoding the polypeptide of interest, at least one stop codon down-stream of the first polynucleotide, and a second polynucleotide downstream of the stop codon encoding an immunoglobulin transmembrane anchor or a functional variant thereof; b) cultivating the eukaryotic host cells to allow expression of the polypeptide of interest such that at least a portion of the polypeptide of interest is expressed as a fusion polypeptide comprising the immunoglobulin transmembrane anchor or a functional variant thereof, wherein said fusion polypeptide is being displayed on the surface of said host cell; c) selecting at least one eukaryotic host cell based upon the presence or amount of the fusion polypeptide displayed on the cell surface. Also provided are host cells, comprising respectively designed heterologous nucleic acids and methods for producing a polypeptide using respective host cells.