发明公开
EP3083951A1 PROCESS FOR PRODUCING A BETA-GLUCAN POLYMER AND GENETICALLY MODIFIED MICROORGANISMS USEFUL IN THIS PROCESS
审中-公开
生产β-葡聚糖聚合物和本方法中有用的遗传修饰的微生物的方法
- 专利标题: PROCESS FOR PRODUCING A BETA-GLUCAN POLYMER AND GENETICALLY MODIFIED MICROORGANISMS USEFUL IN THIS PROCESS
- 专利标题(中): 生产β-葡聚糖聚合物和本方法中有用的遗传修饰的微生物的方法
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申请号: EP14830519.6申请日: 2014-12-12
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公开(公告)号: EP3083951A1公开(公告)日: 2016-10-26
- 发明人: HLUBEK, Andrea , BROCKMANN, Beata , HEROLD, Andrea , GRANSTRÖM, Mari , FLECK, Christian
- 申请人: Wintershall Holding GmbH
- 申请人地址: Friedrich-Ebert-Strasse 160 34119 Kassel DE
- 专利权人: Wintershall Holding GmbH
- 当前专利权人: Wintershall Holding GmbH
- 当前专利权人地址: Friedrich-Ebert-Strasse 160 34119 Kassel DE
- 代理机构: BASF IP Association
- 优先权: EP13198610 20131219
- 国际公布: WO2015091231 20150625
- 主分类号: C12N9/10
- IPC分类号: C12N9/10 ; C12P19/04
摘要:
Process for producing a polymer consisting of a linear main chain of beta-D-(1-3)- glucopyranosyl units having a single beta-D-glucopyranosyl unit (1-6) linked to a beta-D- glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3, said process comprising the steps of: (i) culturing in a medium a genetically modified microorganism capable of producing a polymer consisting of a linear main chain of beta-D-(1-3)-glucopyranosyl units having a single beta-D-glucopyranosylunit (1-6) linked to a beta-D-glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3, wherein the modification confers an increased activity -compared to a non-modified control microorganism of the same strain -of at least two gene products selected from the group consisting of (a) hexokinase, (b) phosphoglucomutase,. (c) UTP-glucose-1-phosphate uridyltrasferase, (d) 1,3–beta-glucan synthase (e) branching enzyme; under conditions allowing said microorganism to produce said polymer; (ii) optionally recovering said polymer from the medium.
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