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    PROCESS FOR PRODUCING A BETA-GLUCAN POLYMER AND GENETICALLY MODIFIED MICROORGANISMS USEFUL IN THIS PROCESS
    2.
    发明公开
    PROCESS FOR PRODUCING A BETA-GLUCAN POLYMER AND GENETICALLY MODIFIED MICROORGANISMS USEFUL IN THIS PROCESS 审中-公开
    生产β-葡聚糖聚合物和本方法中有用的遗传修饰的微生物的方法

    公开(公告)号：EP3083951A1

    公开(公告)日：2016-10-26

    申请号：EP14830519.6

    申请日：2014-12-12

    申请人： Wintershall Holding GmbH

    发明人： HLUBEK, Andrea ,  BROCKMANN, Beata ,  HEROLD, Andrea ,  GRANSTRÖM, Mari ,  FLECK, Christian

    IPC分类号： C12N9/10 ,  C12P19/04

    CPC分类号： C12N9/1051 ,  C12N9/107 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/90 ,  C12P19/04

    摘要： Process for producing a polymer consisting of a linear main chain of beta-D-(1-3)- glucopyranosyl units having a single beta-D-glucopyranosyl unit (1-6) linked to a beta-D- glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3, said process comprising the steps of: (i) culturing in a medium a genetically modified microorganism capable of producing a polymer consisting of a linear main chain of beta-D-(1-3)-glucopyranosyl units having a single beta-D-glucopyranosylunit (1-6) linked to a beta-D-glucopyranosyl unit of the linear main chain with an average branching degree of about 0.3, wherein the modification confers an increased activity -compared to a non-modified control microorganism of the same strain -of at least two gene products selected from the group consisting of (a) hexokinase, (b) phosphoglucomutase,. (c) UTP-glucose-1-phosphate uridyltrasferase, (d) 1,3–beta-glucan synthase (e) branching enzyme; under conditions allowing said microorganism to produce said polymer; (ii) optionally recovering said polymer from the medium.

    摘要翻译： 用于生产由β-D-（1-3） - 吡喃葡萄糖基单元的线性主链组成的聚合物的方法，所述β-D-（1-3） - 吡喃葡萄糖基单元具有与线性的β-D-吡喃葡萄糖基单元连接的单个β-D-吡喃葡萄糖基单元 主链的平均支化度为约0.3，所述方法包括以下步骤：（i）在培养基中培养能够产生由β-D-（1-3）的线性主链组成的聚合物的基因修饰微生物， - 吡喃葡萄糖基单元，其具有与平均支化度为约0.3的线性主链的β-D-吡喃葡萄糖基单元连接的单一β-D-吡喃葡萄糖基单元（1-6），其中所述修饰赋予与 （a）己糖激酶，（b）磷酸葡萄糖变位酶，至少两种基因产物的相同菌株的未修饰的对照微生物。 （c）UTP-葡萄糖-1-磷酸尿苷转移酶，（d）1,3-β-葡聚糖合酶（e）分支酶; 在允许所述微生物产生所述聚合物的条件下; （ii）任选从介质中回收所述聚合物。