发明公开
EP3098322A1 METHOD TO DETECT ACTIVITY OF A POLYMERASE
审中-公开
VERFAHREN ZUR ERKENNUNG DERAKTIVITÄTEINES POLYMERASE
- 专利标题： METHOD TO DETECT ACTIVITY OF A POLYMERASE
- 专利标题（中）： VERFAHREN ZUR ERKENNUNG DERAKTIVITÄTEINES POLYMERASE
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申请号： EP15169966.7申请日： 2015-05-29
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公开(公告)号： EP3098322A1公开(公告)日： 2016-11-30
- 发明人: BERGBREDE, Tim , LARSSON, Nils-Göran , GUSTAFSSON, Claes , FALKENBERG-GUSTAFSSON, Maria
- 申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Lead Discovery Center GmbH
- 申请人地址： Hofgartenstraße 8 80539 München DE
- 专利权人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.,Lead Discovery Center GmbH
- 当前专利权人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.,Lead Discovery Center GmbH
- 当前专利权人地址： Hofgartenstraße 8 80539 München DE
- 代理机构： Arth, Hans-Lothar
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The present invention relates to methods for detection of nucleotide polymerase activity and methods of detecting compounds that modulate nucleotide polymerase activity, by detecting product formation of the nucleotide polymerase to be tested based on determination of close proximity of two labeled nucleotide probes able to bind the product of the nucleotide polymerase. It is preferred that proximity dependent energy transfer, such as förster resonance energy transfer, between said labeled nucleotide probes is determined. The invention further provides kits comprising components for carrying out the inventive methods for detection of nucleotide polymerase activity.
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