发明公开
EP3133157A1 A PROCESS FOR PURIFYING VITAMIN K DEPENDENT PROTEINS
审中-公开
VERFAHREN ZUR REINIGUNG VON维生素K-ABHÄNGIGEN蛋白
- 专利标题： A PROCESS FOR PURIFYING VITAMIN K DEPENDENT PROTEINS
- 专利标题（中）： VERFAHREN ZUR REINIGUNG VON维生素K-ABHÄNGIGEN蛋白
-
申请号： EP16190737.3申请日： 2011-03-30
-
公开(公告)号： EP3133157A1公开(公告)日： 2017-02-22
- 发明人: GILLJAM, Gustav , WINGE, Stefan
- 申请人： Octapharma AG
- 申请人地址： Seidenstrasse 2 8853 Lachen CH
- 专利权人： Octapharma AG
- 当前专利权人： Octapharma AG
- 当前专利权人地址： Seidenstrasse 2 8853 Lachen CH
- 代理机构： Patent- und Rechtsanwälte Ullrich & Naumann
- 优先权： EP10158511 20100330; US282895P 20100416
- 主分类号： C12N9/64
- IPC分类号： C12N9/64
摘要：
A process of manufacturing a prion-free Vitamin K dependent Protein in a purification sequence employing chromatography characterized in that
- at least one chromatography step is performed using a multimodal resin
- providing a fraction containing Vitamin K dependent Protein in an aqueous solution;
- contacting the fraction containing the Vitamin K dependent Protein with a multimodal resin at a pH between 6-9;
- optionally washing the multimodal resin having the Vitamin K dependent Protein adsorbed with an aqueous washing buffer to wash away contaminants and retain the Vitamin K dependent Protein, before the Vitamin K dependent Protein is eluted;
- the Vitamin K dependent Protein is eluting from the multimodal resin at a pH between 6 to 9 in a buffer comprising arginine; and
- optionally collecting Vitamin K dependent Protein containing fractions in purified or enriched form.
- at least one chromatography step is performed using a multimodal resin
- providing a fraction containing Vitamin K dependent Protein in an aqueous solution;
- contacting the fraction containing the Vitamin K dependent Protein with a multimodal resin at a pH between 6-9;
- optionally washing the multimodal resin having the Vitamin K dependent Protein adsorbed with an aqueous washing buffer to wash away contaminants and retain the Vitamin K dependent Protein, before the Vitamin K dependent Protein is eluted;
- the Vitamin K dependent Protein is eluting from the multimodal resin at a pH between 6 to 9 in a buffer comprising arginine; and
- optionally collecting Vitamin K dependent Protein containing fractions in purified or enriched form.
公开/授权文献
- EP3133157B1 A PROCESS FOR PURIFYING VITAMIN K DEPENDENT PROTEINS 公开/授权日：2019-05-08
信息查询
IPC分类:
