本发明涉及包括被设计为产生具有第一光轴（A1）的光片（6）的照明物镜（2）和被设计用于检测来自样品平面（4）的光的检测物镜（3）的显微镜（1） 第二光轴（A2）。 照明透镜（2）和检测目标（3）的相对对齐以彼此和样品平面（4）相交的第一光轴（A1）和在样品平面中的第二光学轴线（A2）（4）和另一个在 基本上包含直角。 光轴（A1，A2）各自包括与垂直于样本平面（4）定向的参考轴（B）的非零角度。 此外，存在设计用于样本平面（4）的宽视野照明的概观照明装置，其包括具有第三光轴（A3）的照明光学单元（9）。 检测物镜（3）的特征在于提供并设计成检测光片（6）的光和照明光学系统（9）的光。 本发明还涉及一种用于操作光学显微镜的方法。

The invention relates to a microscope (1) comprising a for generating a light sheet (6) trained illumination lens (2) having a first optical axis (A1) and a coming for detecting from the sample plane (4) light trained detection lens (3) with a second optical axis (A2). The illumination lens (2) and the detection objective (3) are aligned relative to each other and to the sample plane (4) that intersect the first optical axis (A1) and the second optical axis (A2) in the sample plane (4) and another one in the include substantially a right angle. The optical axes (A1, A2) close to a perpendicular to the sample plane (4) facing the reference axis (B) each have a non-zero angle. Further, a for wide-field illumination of the specimen plane (4) is formed overview lighting apparatus comprising an illumination optical system (9) having a third optical axis (A3) are present. It is characteristic that the detection lens (3) is provided for detecting both light of the light sheet (6) and of light of the illumination optical system (9) and is formed. The invention further relates to a method of operating a light sheet microscope.