- 专利标题: TRANSFORMED FUNGUS HAVING ENHANCED ERGOTHIONEINE PRODUCTIVITY AND METHOD FOR PRODUCING ERGOTHIONEINE
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申请号: EP15880177申请日: 2015-12-25
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公开(公告)号: EP3252142A4公开(公告)日: 2018-06-27
- 发明人: HARA SEIICHI , KUROSAWA KEIKO , ICHIKAWA KEIICHI
- 申请人: KIKKOMAN CORP
- 专利权人: KIKKOMAN CORP
- 当前专利权人: KIKKOMAN CORP
- 优先权: JP2015017328 2015-01-30; JP2015157444 2015-08-07
- 主分类号: C12N1/15
- IPC分类号: C12N1/15 ; C12N9/10 ; C12N15/09 ; C12P13/04 ; C12R1/66 ; C12R1/665 ; C12R1/685 ; C12R1/69
摘要:
The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene (s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5'-phosphate as a coenzyme.
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