Provided is a hydrogel composition including thermosensitive poly(phosphazene) to which a plurality of hydrophobic amino acids, hydrophilic polymers, and host molecules are substituted; and a physiologically active material linked to a guest molecule, wherein the poly(phosphazene) and the physiologically active material form a conjugate by inclusion of the guest molecule in the host molecule via a host-guest interaction.
When injected into a living body, the hydrogel composition of the present disclosure slowly releases the physiologically active material and provides favorable conditions for stem cell differentiation. In addition, the hydrogel composition, in which the kind, ratio, and sequence of the physiologically active material are controlled to be suitable for stem cell differentiation, may be prepared with high reproducibility.