Further provided in embodiments of the present disclosure are a display substrate and a display device. The display substrate comprises a base substrate and a plurality of sub-pixels. The density of sub-pixels of a first display region is less than the density of sub-pixels of a second display region; the plurality of sub-pixels comprises a plurality of first pixel groups and a plurality of second pixel groups, the plurality of first pixel groups are located in the first display region, the plurality of second pixel groups are located in the second display region, the first display region comprises a plurality of sub-display regions and sub-light-transmitting regions located between the plurality of sub-display regions, each of the first pixel groups comprises a first sub-pixel, a second sub-pixel, and a third sub-pixel arranged in sequence along a first direction, each of the second pixel groups comprises a first sub-pixel, a second sub-pixel pair, and a third sub-pixel arranged along the first direction, and the second sub-pixel pair comprises two second sub-pixels arranged along a second direction. The display substrate can lower the resolution of the first display region and increase the service life of the second sub-pixels in the first pixel groups.