- 专利标题: COLD STORAGE SOLUTION AND STORAGE METHOD FOR STEM CELLS
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申请号: EP22807260.9申请日: 2022-03-29
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公开(公告)号: EP4338590A1公开(公告)日: 2024-03-20
- 发明人: AIZAWA Akira , HYON Suong-Hyu , HYON Woogi
- 申请人: BMG INCORPORATED
- 申请人地址: JP Kyoto-shi, Kyoto 601-8023 45, Minamimatsunoki-cho Higashikujo Minami-ku
- 代理机构: Global IP Europe Patentanwaltskanzlei
- 优先权: JP2021080546 20210511
- 国际公布: WO2022239556 20221117
- 主分类号: A01N1/02
- IPC分类号: A01N1/02 ; C12N5/073 ; C12N5/10
摘要:
Provided are a cold storage method and a cold storage liquid that are suitable for non-freezing refrigerated storage of human or animal stem cells such as iPS cells or embryos in a non-frozen state. According to an embodiment, the non-freezing refrigerated storage liquid is: a mixture liquid (HTMα, HTMx2c13), in which MEM-alpha (Minimum Essential Medium Eagle (MEM) Alpha Modification) is mixed with a UW solution (UW_sln; BELZER UW (registered trademark) COLD STORAGE SOLUTION), or a modified UW solution (polymer component being changed to PVA), are mixed in a ratio of about 1/1 to 1/2; or a storage liquid having a composition equivalent to the mixture liquid, especially in respect of concentrations of potassium ions and sodium ions. And, arousal period(s) of maintaining a temperature of 30°C to 37°C is inserted at each of two to five days, into a non-freezing refrigerated storage period, which is made at 2°C to 8°C for 2 to 10 days or 4 to 20 days.
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