发明公开
- 专利标题： SYNTHETIC SPIKE-IN CONTROLS FOR CELL-FREE MEDIP SEQUENCING AND METHODS OF USING SAME
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申请号： EP24177800.0申请日： 2020-11-06
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公开(公告)号： EP4435118A2公开(公告)日： 2024-09-25
- 发明人: WILSON, Samantha L , SHEN, Shu Yi , DINIZ DE CARVALHO, Daniel , HOFFMAN, Michael M. , TRICHE, Timothy J.
- 申请人： University Health Network , Van Andel Research Institute
- 申请人地址： CA Toronto, ON M5G 2C4 190 Elizabeth Street R. Fraser Elliott Building - Room 1S-417
- 专利权人： University Health Network,Van Andel Research Institute
- 当前专利权人： University Health Network,Van Andel Research Institute
- 当前专利权人地址： CA Toronto, ON M5G 2C4 190 Elizabeth Street R. Fraser Elliott Building - Room 1S-417
- 代理机构： Avidity IP
- 优先权： US 1962931411P 2019.11.06
- 分案原申请号： 20884272.4 2020.11.06
- 主分类号： C12Q1/6869
- IPC分类号： C12Q1/6869
摘要：
There is described herein, a method of capturing and analyzing cell-free methylated DNA in a sample. The method involves subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA. A predetermined amount of control synthetic DNA fragments are added to the sample. The control synthetic DNA fragments each have a known nucleic acid sequence that does not align to a target genome sequence, and at least some of the control synthetic DNA fragments are methylated. The sample is denatured, and cell-free methylated DNA and the control synthetic DNA fragments are captured using a binder selective for methylated polynucleotides. The captured DNA is amplified and sequenced.
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