The present disclosure provides a production process of a medium-strength high-conductivity aluminum alloy monofilament and an aluminum alloy monofilament thereof, and relates to the technical field of aluminum alloy materials. The production process comprises the following steps: providing an aluminum alloy rod; drawing the aluminum alloy rod to obtain alloy monofilaments, and performing pre-aging treatment on the alloy monofilaments at 100 to 150°C during the drawing process; and performing artificial aging treatment on the alloy monofilaments to obtain the medium-strength high-conductivity aluminum alloy monofilaments. The present disclosure further provides an aluminum alloy monofilament. The aluminum alloy monofilament obtained through the production process of the medium-strength high-conductivity aluminum alloy monofilament provided in the present disclosure has a strength higher than or equal to 270 MPa, an electrical conductivity higher than or equal to 59% IACS, and an elongation greater than or equal to 5%.