The what is described herein is a method of using a compound which activates the pyruvate kinase. The present invention is a composition comprising a compound as disclosed in an effective amount of (1) herein blood or a pharmaceutically acceptable salt thereof, and a compound or a salt thereof with carriers disclosed herein (2) objects, or (3) comprises contacting the the compound as disclosed herein or a pharmaceutical composition comprising a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier, erythrocytes required ( It provides a method of increasing the life of RBCs).