The present invention has the following structure: A nucleic acid construct comprising a A-X-B, wherein, A and B is a nucleic acid sequence encoding the first and second polypeptide of interest (POI) ; X is a nucleic acid sequence encoding a cleavage site, either the first or the second POI is a transmembrane protein containing an intracellular retention signal, provides a nucleic acid construct. In one aspect, the transmembrane protein comprises an end domain comprising the intracellular retention signal. In another aspect, the intracellular retention signal directs so as not directed to the secretory pathway of the transmembrane protein. In yet another aspect, the intracellular retention signal directs the transmembrane protein to the membrane bound subcellular compartments.