The present invention provides a bioconjugation complex formed by covalently bonding a linker compound including a calcium phosphate-based bioceramic, an amino group and a carboxylate group, and a hydrophobic protein, and a method for preparing the same. That is, the present invention provides an effective manufacturing method for covalently conjugating a bioceramic hydroxyapatite and a protein by using a peptide-coupling agent.
A peptide-coupling agent can be used to directly conjugate a protein other than a hydrophobic protein to a bioceramic, and in the case of a hydrophobic protein, a bioceramic and a linker, or a bioceramic and linker conjugate and a biocatalyst can be combined by using a peptide-coupling agent. Bonding efficiency can be improved. In addition, by using a linker compound, a hydrophobic biocatalyst can be easily bonded to the polar bioceramic surface. The bioconjugation complex prepared by the above manufacturing method is covalently bonded to the bioceramic surface of functional proteins including hydrophobic proteins, so it can be applied to a support for tissue engineering suitable for a living body, a material for inducing and shielding tissue regeneration, and a biological cell proliferation material. have.