- 专利标题： Cell-free protein expression using rolling circle amplification product
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申请号： US15145838申请日： 2016-05-04
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公开(公告)号： US10077459B2公开(公告)日： 2018-09-18
- 发明人: John Richard Nelson , Robert Scott Duthie , Erik Leeming Kvam , Wei Gao
- 申请人： General Electric Company
- 申请人地址： US NY Schenectady
- 专利权人： GENERAL ELECTRIC COMPANY
- 当前专利权人： GENERAL ELECTRIC COMPANY
- 当前专利权人地址： US NY Schenectady
- 代理机构： GE Global Patent Operation
- 代理商 Pabitra Chakrabarti
- 主分类号： C12P21/00
- IPC分类号： C12P21/00 ; C12N15/63 ; C12N15/67
摘要：
Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
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