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公开(公告)号：US10000742B2
公开(公告)日：2018-06-19
申请号：US14945483
申请日：2015-11-19
CPC分类号： C12N7/00 , B01L3/508 , B01L2200/082 , C12N15/1017 , C12N2760/14111 , C12N2760/14151 , C12N2760/14163 , C12Q1/6806 , C12Q1/70 , C12Q1/701 , C12Q2523/113 , C12Q2523/308 , C12Q2527/125
摘要： The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.
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公开(公告)号：US09279150B2
公开(公告)日：2016-03-08
申请号：US13840062
申请日：2013-03-15
CPC分类号： C12N9/22 , C07K1/00 , C07K14/00 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Y301/26 , C12Q2521/301 , C12Q2525/101 , C12Q2537/1376 , C12Q2527/101
摘要： Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
摘要翻译： 本文提供了能够切割含有肌苷的DNA序列的突变体内切核酸酶V酶。 还描述了使用这种突变体内切核酸酶V酶将切口引入到包括一种或多种肌苷的靶DNA中并使用DNA聚合酶产生靶DNA扩增子的核酸测定法和试剂。
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公开(公告)号：US20160002621A1
公开(公告)日：2016-01-07
申请号：US14321160
申请日：2014-07-01
发明人： John Richard Nelson , David Roger Moore , Robert Scott Duthie , Matthew Jeremiah Misner , Gregory Andrew Grossmann , Elizabeth Marie Dees , Patrick McCoy Spooner , Erik Leeming Kvam , Andrew Arthur Paul Burns , Vicki Herzl Watkins
IPC分类号： C12N15/10
CPC分类号： C12N15/1006 , C12Q1/6834 , C12Q2565/625
摘要： A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.
摘要翻译： 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 并使核酸扩增反应混合物通过样品施用区跨越一定长度的底物扩增形成核酸扩增产物的靶核酸; 其中具有第一分子量的靶核酸基本上固定在样品施用区，并且其中具有第二分子量的扩增产物从样品施用区迁移。 还提供了相关联的设备。
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4.发明申请ENDONUCLASE-ASSISTED ISOTHERMAL AMPLIFICATION USING CONTAMINATION-FREE REAGENTS 审中-公开使用无污染试剂进行内切酶辅助同位素放大公开(公告)号：US20130323795A1
公开(公告)日：2013-12-05
申请号：US13965696
申请日：2013-08-13
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/6848 , C12Q2521/101 , C12Q2521/307 , C12Q2525/101 , C12Q2525/125 , C12Q2531/119 , C12Q2521/319
摘要： Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.
摘要翻译： 公开了使用用核酸酶预处理的去污底漆溶液进行内切核酸酶辅助的DNA扩增反应的方法和试剂盒。 还公开了使用含核酸酶抗性，含肌苷的引物，内切核酸酶V酶将包含至少一种肌苷的靶DNA引入切口的核酸扩增测定法和DNA聚合酶以产生靶DNA扩增子。
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5.发明申请公开(公告)号：US20180305718A1
公开(公告)日：2018-10-25
申请号：US15491125
申请日：2017-04-19
发明人： John Richard Nelson , Robert Scott Duthie , Patrick McCoy Spooner , John Anthony Schiel , Lisa Anne Lowery , Anja Josifa Smith
CPC分类号： C12N15/907 , C12N15/102 , C12N15/11 , C12N2310/20
摘要： A method of site-specific modification of an endogenous target DNA of a eukaryotic cell is provided. The method includes contacting the endogenous target DNA having an intended modification site with (i) a gene editing system configured to introduce a double strand break in the endogenous target DNA at or near the intended modification site, and (ii) a donor DNA repair template comprising a plurality of tandem repeat sequences. In the method, each of the plurality of tandem repeat sequences comprises an exogenous donor DNA sequence flanked by a donor 5′ flanking sequence and a donor 3′ flanking sequence. The donor 5′ flanking sequence and the donor 3′ flanking sequence are homologous to a continuous DNA sequence on either side of the intended modification site in the endogenous target DNA.
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公开(公告)号：US10077459B2
公开(公告)日：2018-09-18
申请号：US15145838
申请日：2016-05-04
CPC分类号： C12P21/00 , C12N15/63 , C12N15/67 , C12Q2531/125
摘要： Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
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公开(公告)号：US20170321239A1
公开(公告)日：2017-11-09
申请号：US15145838
申请日：2016-05-04
IPC分类号： C12P21/00
CPC分类号： C12P21/00 , C12N15/63 , C12N15/67 , C12Q2531/125
摘要： Methods for in vitro transcription and translation from an RCA product are provided. The methods comprise providing a double-stranded RCA product, wherein the double-stranded RCA product consists essentially of tandem repeats of a minimalistic expression sequence. The methods further comprise expressing a protein from the double-stranded RCA product in a cell-free expression system.
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公开(公告)号：US20190062795A1
公开(公告)日：2019-02-28
申请号：US15941057
申请日：2018-03-30
IPC分类号： C12P19/34 , C12Q1/6848 , C12Q1/6844
摘要： Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.
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公开(公告)号：US20180001231A1
公开(公告)日：2018-01-04
申请号：US15209411
申请日：2016-07-13
发明人： Christopher Michael Puleo , Craig Patrick Galligan , Gregory Andrew Grossmann , Erik Leeming Kvam , Robert Scott Duthie , Kenneth Wayne Rigby , Paul Michael Smigelski, JR. , Victoria Eugenia Cotero , Jason William Castle , John Donald Burczak , James Edward Rothman
摘要： A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.
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公开(公告)号：US20160002715A1
公开(公告)日：2016-01-07
申请号：US14321235
申请日：2014-07-01
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q2531/119 , C12Q2531/125 , C12Q2565/518
摘要： A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; applying an aqueous buffer to the sample application zone of the substrate to washes away one or more inhibitors present on the sample application zone; and applying an isothermal nucleic acid amplification reaction mixture to the sample application zone to amplify the target nucleic acid to form a nucleic acid amplification product. The target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight.
摘要翻译： 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 将水性缓冲液施加到基材的样品施加区域以洗涤存在于样品施加区上的一种或多种抑制剂; 以及将等温核酸扩增反应混合物应用于样品施用区域以扩增靶核酸以形成核酸扩增产物。 具有第一分子量的靶核酸基本上固定在样品施用区，并且其中扩增产物具有第二分子量。
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