Technologies for end-to-end biometric-based authentication and locality assertion include a computing device with one or more biometric devices. The computing device may securely exchange a key between a driver and a secure enclave. The driver may receive biometric data from the biometric sensor in a virtualization-protected memory buffer and encrypt the biometric data with the shared key. The secure enclave may decrypt the biometric data and perform a biometric authentication operation. The computing device may measure a virtual machine monitor (VMM) to generate attestation information for the VMM. A secure enclave may execute a virtualization report instruction to request the attestation information. The processor may copy the attestation information into the secure enclave memory. The secure enclave may verify the attestation information with a remote attestation server. If verified, the secure enclave may provide a shared secret to the VMM. Other embodiments are described and claimed.