An array substrate and a manufacturing method thereof, and a display device are provided. The array substrate includes: a base substrate, an active layer, and a first polarization structure. The active layer is disposed on the base substrate; the first polarization structure is disposed on a side of the active layer facing the base substrate, and an orthographic projection of the first polarization structure on the base substrate is at least partially overlapped with an orthographic projection of the active layer on the base substrate.