A display panel, a driving method thereof, a manufacturing method thereof and a display device. The display panel includes a first emission layer (EML) and a second EML. The first EML includes a plurality of first display pixel, the first display pixels each include at least one first luminescent unit; the second EML is disposed at a light-emitting side of the first display pixels and includes a plurality of second display pixels, the second display pixels each include at least one second luminescent unit, the second EML includes light-transmitting regions and light-shielding regions alternately arranged in a row direction; the first display pixels and orthographic projections of the second display pixels on the first EML are partially overlapped and the first display pixels are partially exposed from corresponding light-transmitting regions; and the first and second display pixels have a same light-emitting direction.