The present invention discloses a position calibration method, a test circuit board, a sample panel and a position calibration apparatus. The position calibration method comprises: Step S1, providing a test circuit board and forming at least one first sample connecting finger; Step S2, making a sample panel comprising second sample connecting fingers corresponding to each first sample connecting finger; Step S3, bringing the test circuit board into contact with the sample panel; and Step S4, detecting whether each first sample connecting finger is electrically connected to a corresponding second sample connecting finger, and when any one of the first sample connecting fingers is detected as in disconnection with the corresponding second sample connecting finger, adjusting a position of the test circuit board and/or the sample panel.