A pixel definition structure, an organic light-emitting device and the encapsulation method thereof, and a display apparatus are provided, in the field of display technology. The pixel definition structure includes a plurality of barriers, wherein the plurality of barriers includes a first barrier and a second barrier. The height of the first barrier is smaller than that of the second barrier, and a barrier at a first edge area of the pixel definition structure is the first barrier. The present disclosure can solve the problem of the poor encapsulation effect and improve the encapsulation effect. The present disclosure is applied to an organic light-emitting device.