A pouch cleaning assembly that includes a treatment chamber and a guide assembly. The treatment chamber includes an elongated central channel extending therethrough. The guide assembly having a first side guide bar and a second side guide bar. The guide bars defining a channel therebetween that is structurally configured to facilitate the retention of spouts of a plurality of pouches in slidable engagement along the first and second guide bar. Any abutment of adjacent portions of the plurality of pouches within the treatment chamber define relative points of contact. The channel is positioned within the elongated central channel, wherein the channel is undulating in at least one of a vertical and a horizontal direction along the length thereof sufficient so as to substantially expose the relative points of contact between adjacent ones of the plurality of spouts so as to expose the same to the cleaning preparation.