Provided are a display substrate and a method of fabricating the same. The display substrate includes: a substrate including a display area and a first non-display area and a second non-display area which are located around the display area; first signal transmission portions located in the first non-display area; second signal transmission portions located in the second non-display area; and a seal line including a first seal line pattern disposed on the first signal transmission portions and a second seal line pattern disposed on the second signal transmission portions, wherein each of the first signal transmission portions includes a gate wiring and a step compensation portion which is disposed on the gate wiring and under the first seal line pattern, and the first seal line pattern overlaps the step compensation portion.