Invention Grant
- Patent Title: Methods of lowering the error rate of massively parallel DNA sequencing using duplex consensus sequencing
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Application No.: US15660785Application Date: 2017-07-26
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Publication No.: US10287631B2Publication Date: 2019-05-14
- Inventor: Jesse Salk , Lawrence A. Loeb , Michael Schmitt
- Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
- Applicant Address: US WA Seattle
- Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
- Current Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
- Current Assignee Address: US WA Seattle
- Agency: Perkins Coie LLP
- Agent Lara J. Dueppen
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/6876 ; C12Q1/6869 ; C12Q1/6806

Abstract:
Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand.
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