公开(公告)号：US12297483B2

公开(公告)日：2025-05-13

申请号：US17485813

申请日：2021-09-27

Applicant: University of Washington through its Center for Commercialization ,  PerkinElmer Health Sciences, Inc.

Inventor： Alexander Cherkassky ,  Jason Cournoyer ,  Michael Gelb

IPC: C12Q1/34 ,  C07C233/18 ,  C07H15/10

Abstract: Provided are molecules and methods for detecting enzymatic activity of various lysosomal storage enzymes. The molecules may be used as internal standards that may be combined with substrates that have improved solubility.

公开(公告)号：US12258629B2

公开(公告)日：2025-03-25

申请号：US16503398

申请日：2019-07-03

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6876

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US20250019761A1

公开(公告)日：2025-01-16

申请号：US18648154

申请日：2024-04-26

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US12167864B1

公开(公告)日：2024-12-17

申请号：US18151013

申请日：2023-01-06

Applicant: University of Washington through its Center for Commercialization

Inventor： Adam D. Maxwell ,  Bryan W. Cunitz ,  Wayne Kreider ,  Oleg A. Sapozhnikov ,  Ryan S. Hsi ,  Michael R. Bailey

IPC: A61B17/22

Abstract: A method for attempting to fragment or comminute an object in a body using ultrasound includes producing a burst wave lithotripsy (BWL) waveform by a therapy transducer. The BWL waveform is configured to fragment or comminute the object. The BWL waveform includes a first burst of continuous ultrasound cycles and a second burst of continuous ultrasound cycles. A burst frequency corresponds to a frequency of repeating the bursts of the BWL waveform. The method also includes determining a cycle frequency f of the continuous ultrasound cycles within the first burst and the second burst based on a target fragment size D, where the cycle frequency is: f(MHz)=0.47/D(mm).

公开(公告)号：US12042531B2

公开(公告)日：2024-07-23

申请号：US17451607

申请日：2021-10-20

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Mary L. Disis ,  Denise Cecil ,  Meredith Slota

IPC: C12N15/00 ,  A61K39/00 ,  C07K14/47 ,  C07K14/705 ,  C07K14/71 ,  C12N9/10 ,  C12N15/62 ,  A61K48/00

CPC classification number: A61K39/0011 ,  A61K39/001103 ,  A61K39/001106 ,  A61K39/001129 ,  A61K39/00115 ,  A61K39/001152 ,  C07K14/4702 ,  C07K14/705 ,  C07K14/70567 ,  C07K14/70596 ,  C07K14/71 ,  C12N9/104 ,  C12N15/62 ,  C12Y203/02 ,  A61K2039/53 ,  A61K2039/54 ,  A61K2039/572 ,  A61K48/00

Abstract: The compositions described herein include an epitope of a peptide that may elicit an immune response in a subject following administration. The compositions may comprise nucleic acids. The compositions may comprise peptides. The methods described herein include administering a composition comprising an epitope of a peptide to a subject in need thereof.

公开(公告)号：US20240207378A1

公开(公告)日：2024-06-27

申请号：US18485536

申请日：2023-10-12

Applicant: University of Washington through its Center for Commercialization

Inventor： David W. Russell ,  Roli K. Hirata

IPC: A61K39/00 ,  A61K35/12 ,  A61K35/28 ,  C07K14/74

CPC classification number: A61K39/0005 ,  A61K35/28 ,  C07K14/70539 ,  A61K35/12 ,  A61K2039/515 ,  C07K2319/00 ,  C12N2740/17043 ,  C12N2750/14143 ,  C12N2800/30

Abstract: The invention provides isolated primate cells preferably human cells that comprise a genetically engineered disruption in a beta-2 microglobulin (B2M) gene, which results in deficiency in MHC class I expression and function. Also provided are the method of using the cells for transplantation and treating a disease condition.

公开(公告)号：US11993815B2

公开(公告)日：2024-05-28

申请号：US17392175

申请日：2021-08-02

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6876

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2525/185 ,  C12Q2525/191 ,  C12Q2535/119 ,  C12Q1/6806 ,  C12Q2525/191 ,  C12Q2535/119 ,  C12Q2535/122 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US20240084385A1

公开(公告)日：2024-03-14

申请号：US18465952

申请日：2023-09-12

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Jesse Salk ,  Lawrence A. Loeb ,  Michael Schmitt

IPC: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

公开(公告)号：US20240083953A1

公开(公告)日：2024-03-14

申请号：US18475950

申请日：2023-09-27

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Andre LIEBER ,  Hongjie WANG

IPC: C07K14/005 ,  A61K47/42 ,  C07K7/06 ,  C12N5/077 ,  C12N7/00 ,  C12N15/10 ,  C12N15/86 ,  G01N33/94

CPC classification number: C07K14/005 ,  A61K47/42 ,  C07K7/06 ,  C12N5/0652 ,  C12N7/00 ,  C12N15/10 ,  C12N15/86 ,  G01N33/94 ,  A61K38/00 ,  C12N2710/10321 ,  C12N2710/10322

Abstract: Disclosed are recombinant adenoviral compositions and methods for their use in treating disorders associated with epithelial tissues.

公开(公告)号：US11821033B2

公开(公告)日：2023-11-21

申请号：US17111167

申请日：2020-12-03

Applicant: University of Washington through its Center for Commercialization

Inventor： Jens H. Gundlach ,  Andrew Laszlo

IPC: C12Q1/6869

CPC classification number: C12Q1/6869 ,  C12Q1/6869 ,  C12Q2523/31 ,  C12Q2565/631

Abstract: The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.