The present disclosure discloses a display panel, a display device and a driving method thereof. The display panel comprises: a first substrate and a second substrate arranged opposite to each other, and liquid crystals disposed between the first substrate and the second substrate, the first substrate comprising a plurality of gate lines and a plurality of data lines, wherein the first substrate is provided with a plurality of pixel regions distributed in an array, each of the plurality of pixel regions being provided with liquid crystals having four initial pretilt angles and comprising a first sub-pixel region and a second sub-pixel region arranged in a scanning direction, each of the first sub-pixel region and the second sub-pixel region being provided with one thin film transistor; each of the plurality of gate lines is connected to gates of all the thin film transistors in a row of pixel regions, each of the plurality of data lines being connected to a first electrode of all the thin film transistors in a column of pixel regions, the first electrode being one of a source and a drain; and the display panel further comprises a driving circuit for providing different gate turn-on voltages to two adjacent gate lines.