The present disclosure relates to a module for regenerating an electrolyte capable of being used in a flow battery, and a method for regenerating an electrolyte of a flow battery using the same, and in particular, to a module for regenerating an electrolyte introducing any one of anode and cathode electrolytes each stored in anode and cathode electrolyte storage units to a counter electrolyte storage unit, and circulating any one of the anode and cathode electrolytes in a direction opposite to the direction of electrolyte introduction to uniformly mix the anode and cathode electrolytes, and as a result, capable of recovering battery capacity reduced by a membrane permeation phenomenon between the anode and cathode electrolytes when driving a flow battery for a long period of time, and a method for regenerating an electrolyte of a flow battery using the same.