Systems and methods corresponding to a peripheral interface service that facilitates independent connection of peripheral devices to virtual desktop instances. The peripheral devices may utilize a network connection facilitated through a local computing device or have direct network communication capabilities. The peripheral interface service can facilitate configuration of peripheral devices. The peripheral interface service can then store the configuration information for processing subsequent connection requests from one or peripheral devices. Additionally, the peripheral interface service can facilitate connection requests for configured peripheral devices. Based on processing the request and identification of a virtual desktop instance, the peripheral interface service can then facilitate the connection request to the identified virtual desktop instance.