A two-stage operational amplifier is provided to comprise a bias voltage generator, a first stage operational amplifier and a second stage operational amplifier, wherein the first stage operational amplifier comprises a folded cascode amplifier circuit and a cross coupling load, the cross coupling load is coupled to a load differential pair in the folded cascode amplifier circuit, the cross coupling load comprises two transistors, the two transistors in the cross coupling load and two transistors in the load differential pair constitute two current mirror structures, which are cross coupled. In the solution, the cross coupling load is added to the load differential pair in the folded cascode amplifier circuit, to increase gain of the two-stage operational amplifier by using positive feedback and negative conductance gain enhancement technology; while parameters of MOSFETs in the folded cascode amplifier circuit are properly set to reduce noise of the two-stage operational amplifier.