Invention Grant
- Patent Title: Methods for full-length amplification of double-stranded linear nucleic acids of unknown sequences
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Application No.: US15037322Application Date: 2014-12-14
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Publication No.: US10407720B2Publication Date: 2019-09-10
- Inventor: Kuo Ping Chiu , Yu-Shin Nai
- Applicant: ACADEMIA SINICA
- Applicant Address: TW Taipei
- Assignee: Academia Sinica
- Current Assignee: Academia Sinica
- Current Assignee Address: TW Taipei
- Agency: Intellectual Property Connections, Inc.
- Agent Hsiu-Ming Saunders
- International Application: PCT/US2014/070226 WO 20141214
- International Announcement: WO2015/089496 WO 20150618
- Main IPC: C12Q1/686
- IPC: C12Q1/686 ; G16B30/00 ; C12Q1/6869 ; C12Q1/6886 ; C12Q1/6855 ; G16B35/00 ; G16C20/60

Abstract:
An adaptor for use in amplifying all linear, double-stranded nucleic acid molecules of unknown sequences in a sample is disclosed. The adaptor consists of: (1) the first oligonucleotide (P-oligo) with a phosphate at the 5′ end and without an additional thymine nucleotide at the 3′ end; and (2) the second oligonucleotide (T˜oligo) with an extra 3′-T and without a 5′-phosphate. The P-oligo and T-oligo are complementary to each other except at the 3′-T (thymine) in the T-oligo. The adaptor is ligated to nucleic acids of unknown sequences which have an extra A in the 3′ end (3′˜A overhang) to form adaptor-ligated target nucleic acids. The T-oligo is then employed as a single primer for T-oligo-primed polymerase chain reaction (TOP-PCR) and amplifies the nucleic acids of unknown sequences in full-length.
Public/Granted literature
- US20160298172A1 METHODS FOR FULL-LENGTH AMPLIFICATION OF DOUBLE-STRANDED LINEAR NUCLEIC ACIDS OF UNKNOWN SEQUENCES Public/Granted day:2016-10-13
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