- 专利标题: Selective detection of lactic acid and/or acetic acid bacteria or of fungi
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申请号: US15731735申请日: 2016-01-25
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公开(公告)号: US10415074B2公开(公告)日: 2019-09-17
- 发明人: Charles Cervin
- 申请人: Charles Cervin
- 申请人地址: FR Commercy
- 专利权人: Charles Cervin
- 当前专利权人: Charles Cervin
- 当前专利权人地址: FR Commercy
- 代理机构: Grant Patent Services
- 代理商 Jonathan E. Grant
- 优先权: FR1500163 20150128
- 国际申请: PCT/FR2016/000011 WO 20160125
- 国际公布: WO2016/120534 WO 20160804
- 主分类号: C12Q1/00
- IPC分类号: C12Q1/00 ; C12N1/00 ; C12Q1/20 ; C12Q1/04 ; C12Q1/66 ; G01N21/76 ; G01N33/14
摘要:
A method for detecting lactic acid and/or acetic acid bacteria in a food-processing matrix is taught, using microbial flora including a lactic acid and/or acetic acid bacterial flora and a fungal flora. The bacterial flora contains an adenosine triphosphate of bacterial origin, and the fungal flora contains an adenosine triphosphate of fungal origin. The method comprises applying, to the matrix, before a first time limit, an antifungal having an antifungal action which is lethal, on the fungal flora, and at a second time limit, an antibiotic action which is non lethal, at a second time limit after the first time limit, on the bacterial flora. The microbial flora is detected between the first time limit and the second time limit; the lethal antifungal action releases, into the matrix, for the first time limit, adenosine triphosphate of fungal origin and in which the microbial flora is detected between the first time limit and the second time limit.
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