发明授权
- 专利标题： Methods and compositions for targeted genetic modification using paired guide RNAs
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申请号： US14948221申请日： 2015-11-20
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公开(公告)号： US10457960B2公开(公告)日： 2019-10-29
- 发明人: David Frendewey , Ka-Man Venus Lai , Wojtek Auerbach , Gustavo Droguett , Anthony Gagliardi , David M. Valenzuela , Vera Voronina , Lynn Macdonald , Andrew J. Murphy , George D. Yancopoulos
- 申请人： Regeneron Pharmaceuticals, Inc.
- 申请人地址： US NY Tarrytown
- 专利权人： Regeneron Pharmaceuticals, Inc.
- 当前专利权人： Regeneron Pharmaceuticals, Inc.
- 当前专利权人地址： US NY Tarrytown
- 代理机构： Alston & Bird, LLP
- 代理商 Yongjin Choi
- 主分类号： C12N15/85
- IPC分类号： C12N15/85 ; C12N15/90 ; C12Q1/6888 ; A01K67/027 ; C12N9/22 ; C12N15/10
摘要：
Compositions and methods are provided for creating and promoting biallelic targeted modifications to genomes within cells and for producing non-human animals comprising the modified genomes. Also provided are compositions and methods for modifying a genome within a cell that is heterozygous for an allele to become homozygous for that allele. The methods make use of Cas proteins and two or more guide RNAs that target different locations within the same genomic target locus. Also provided are methods of identifying cells with modified genomes.
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