Invention Grant
- Patent Title: System and method for determining copies-per-unit-volume using PCR and flow control of droplets
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Application No.: US15080801Application Date: 2016-03-25
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Publication No.: US10557174B2Publication Date: 2020-02-11
- Inventor: Gordon A. Janaway , Mark Andersen , Kornelija Zgonc , Michael C. Pallas , Marcin Sikora , Casey R. McFarland , Ferrier N. Le , Haopeng Wang , Jian Gong , Gothami Padmabandu
- Applicant: LIFE TECHNOLOGIES CORPORATION
- Applicant Address: US CA Carlsbad
- Assignee: Life Technologies Corporation
- Current Assignee: Life Technologies Corporation
- Current Assignee Address: US CA Carlsbad
- Main IPC: C12Q1/6886
- IPC: C12Q1/6886 ; C12Q1/686 ; G01N21/64

Abstract:
Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.
Public/Granted literature
- US20160208342A1 SYSTEM AND METHOD FOR DETERMINING COPIES-PER-UNIT-VOLUME USING PCR AND FLOW CONTROL OF DROPLETS Public/Granted day:2016-07-21
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